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Bibliographie de l'Institut Maurice-Lamontagne
Poissons / Parophrys vetulus / Carlottin anglais
MALTAIS, D., R.L. ROY, C.M. COUILLARD, 2010. Hybrid ELISAs for vitellogenins of the endangered Copper Redhorse (Moxostoma hubbsi) and the Shorthead Redhorse (Moxostoma macrolepidotum (Cypriniformes, catostomidae). Ecotoxicol. Environ. Saf., 73(5): 883-892.
[Résumé disponible seulement en anglais]
The shorthead redhorse (sr) Moxostoma macrolepidotum is endemic in North America, while in Canada the copper redhorse (cr) Moxostoma hubbsi is classified as endangered. A commercial heterologous carp vitellogenin (VTG) sandwich ELISA underestimates VTGs in these species. Hybrid ELISAs, using anti-carp VTG antibodies and purified sr- and cr-VTGs as standards, were validated to measure VTGs in plasma and surface mucus of redhorse species. The hybrid ELISAs showed good precision, sensitivity and accuracy. Following induction with β-estradiol 3-benzoate (E2B), levels of plasma VTG (mean ± SEM) in male sr increased from 2.7 ± 1.5 μg/ml to 16.5±2.2 mg/ml. In immature cr, plasma VTG increased to 70.1±13.8 mg/ml after induction, from an initial value of 37.7±51.0 μg/ml. Levels of VTG in mucus from E2B-injected fish were highly correlated (p<0.0001) with levels in plasma, but not in a non-induced mature female. Hybrid assays are a good compromise between homologous and heterologous assays, especially for small-bodied or endangered fish species. The performance of the hybrid assays strongly suggests they could be used to determine exposure of these species to estrogenic contaminants and contribute to restoration efforts of the copper redhorse. Crown Copyright ©2010 Elsevier Inc.
COUILLARD, C.M., B. LAPLATTE, É. PELLETIER, 2009. A fish bioassay to evaluate the toxicity associated with the ingestion of benzo[a]pyrene-contaminated benthic prey. Environ. Toxicol. Chem., 28(4): 772-781 .
[Résumé disponible seulement en anglais]
A bioassay was developed to assess the toxic effects of ingested prey contaminated by polycyclic aromatic hydrocarbons (PAHs) using the teleost Fundulus heteroclitus as a predator and the polychaete Nereis virens as a benthic vector. Ten groups of nine male adult Fundulus were exposed for 21 d to 10 different diets of Nereis contaminated with benzo[a]pyrene (BaP) by spiking dead Nereis with BaP (spiked Nereis [SN] diets, 0–26 μg of BaP per gram dry wt) or by exposing living Nereis to a diet, to sediments, or to both contaminated with BaP (exposed Nereis [EN] diets, 0–16 μg/g dry wt). Another group was exposed to commercial fish food, used as reference diet. Condition and prevalence of histopathological changes were not affected. Exposure to the SN diets containing at least 3.5 μg of BaP per gram dry weight caused an induction of ethoxyresorufin-O-deethylase activity in the intestine but not in the liver. In contrast, fish exposed to the highest doses (≥13.4 μg of BaP per gram dry wt) had increased cellular proliferation rate in the liver but not in the intestine. Quantifiable levels of free BaP tetrol–like metabolites were detected in the bile of fish exposed to diets containing more than 6.8 μg/g dry weight of BaP, and exhibited a dose–response relationship in fish exposed to SN diets. For a similar dose of BaP, EN and SN diets had similar effects. Thus, the BaP metabolic products that could have been produced in Nereis apparently did not contribute to the biomarkers responses. This bioassay can be applied to a variety of prey contaminated naturally or experimentally with PAHs. The present study supports the use of intestinal biomarkers, in addition to hepatic biomarkers, in environmental monitoring to assess the impact of dietary exposure to PAHs.©2009 SETAC
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