ROY, R.L., Y. MORIN, S.C. COURTENAY, P. ROBICHAUD, 2004. Purification of vitellogenin from smooth flounder (Pleuronectes putnami) and measurement in plasma by homologous ELISA. Comp. Biochem. Physiol., Part B, 139: 235-244 .

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Male smooth flounder (Pleuronectes putnami) were induced to produce vitellogenin (VTG) by injection of 17β-estradiol (E2). Anion exchange chromatography of precipitated plasma from E2-injected resulted in a single peak consisting of VTG. Smooth flounder VTG has an approximate molecular mass of ˜520 kDa, determined by gel filtration with molecular weight standards. Purified VTG was used to develop a homologous enzyme-linked immunosorbent assay (ELISA). The flounder VTG ELISA is an indirect antigen competition assay with a detection limit of 15 ng·ml^-1 and a useful range of 30-950 ng·ml^-1 of diluted sample. Intra- and inter-assay precision (as  %CV, n=7) ranged from 1.3 % to 6.0 % and 5.1 %, respectively. The ELISA was evaluated using plasma samples collected from a smooth flounder population captured in the Saint Lawrence Estuary. The ELISA is sensitive enough to differentiate males and non-vitellogenic females from vitellogenic individuals during early vitellogenesis.©2004 Elsevier Inc.