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Bibliography of the Maurice Lamontagne Institute

Robert L. ROY

TREMBLAY-BOURGEOIS, S., N.R. LE FRANÇOIS, R.L. ROY, T.J. BENFEY, A.K. IMSLAND, 2010. Effect of rearing density on the growth and welfare indices of juvenile Spotted Wolffish, Anarhichas minor (Olafsen). Aquacult. Res., 41: 1179-1189 .

The goal of this study was to determine the optimal stocking density for rearing juvenile spotted wolffish, Anarhichas minor (Olafsen), at two different sizes and assess the welfare status in relation to density. No major growth impairment was observed, although smaller fish (50–100 g) were significantly affected by density during the 120 days of the experiment, with final mean weights of 119.6 ± 11.6, 118.0 ± 5.8 and 88.7 ± 0.6 g for initial rearing densities of 10, 20 and 40 kg m-2 respectively. No effect of rearing density was seen for larger fish (100–160 g) during the 90 days of the experiment, with final mean weights of 160.2 ± 5.9, 159.7 ± 3.7 and 163.7 ± 11.5 g at fixed rearing densities of 20, 30 and 40 kg m-2 respectively. Our results suggest that the optimal rearing density of juvenile spotted wolffish is below 40 kg m-2 for smaller size fish (˜50–100 g) and probably ≥40 kg m-2 for the larger fish (100–160 g). Furthermore, it appears that the range of rearing density used did not have a significant effect on a selection of stress indicators (Na+ and K+ concentration, haematocrit, hepatosomatic index, total amount of plasma proteins and liver and muscle water content) and immunity response (plasma lysozyme activity) of juvenile spotted wolffish, making it a very tolerant species to crowding.©2009 Blackwell Publishing Ltd.

MALTAIS, D., R.L. ROY, C.M. COUILLARD, 2010. Hybrid ELISAs for vitellogenins of the endangered Copper Redhorse (Moxostoma hubbsi) and the Shorthead Redhorse (Moxostoma macrolepidotum (Cypriniformes, catostomidae). Ecotoxicol. Environ. Saf., 73(5): 883-892 .

The shorthead redhorse (sr) Moxostoma macrolepidotum is endemic in North America, while in Canada the copper redhorse (cr) Moxostoma hubbsi is classified as endangered. A commercial heterologous carp vitellogenin (VTG) sandwich ELISA underestimates VTGs in these species. Hybrid ELISAs, using anti-carp VTG antibodies and purified sr- and cr-VTGs as standards, were validated to measure VTGs in plasma and surface mucus of redhorse species. The hybrid ELISAs showed good precision, sensitivity and accuracy. Following induction with β-estradiol 3-benzoate (E2B), levels of plasma VTG (mean ± SEM) in male sr increased from 2.7 ± 1.5 μg/ml to 16.5±2.2 mg/ml. In immature cr, plasma VTG increased to 70.1±13.8 mg/ml after induction, from an initial value of 37.7±51.0 μg/ml. Levels of VTG in mucus from E2B-injected fish were highly correlated (p<0.0001) with levels in plasma, but not in a non-induced mature female. Hybrid assays are a good compromise between homologous and heterologous assays, especially for small-bodied or endangered fish species. The performance of the hybrid assays strongly suggests they could be used to determine exposure of these species to estrogenic contaminants and contribute to restoration efforts of the copper redhorse. Crown Copyright ©2010 Elsevier Inc.

MALTAIS, D., R.L. ROY, 2009. Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum)and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody. Fish. Physiol. Biochem., 35: 241-254 .

Vitellogenin (VTG), the egg yolk precursor protein, was purified from plasma of estradiol-3- benzoate (E2B)-treated male shorthead redhorse (Moxostoma macrolepidotum) and immature copper redhorse (Moxostoma hubbsi) by a two-step chromatographic procedure without precipitation. Intact VTGs appeared as dimers with apparent molecular masses, determined by gel filtration, of ˜425 kDa (copper redhorse) and ˜450 kDa (shorthead redhorse). In native polyacrylamide gel electrophoresis (PAGE), dimeric redhorse VTGs appeared as a 520 kDa band. Both VTGs were reduced to a single monomer of ˜150 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and nonreducing conditions, indicating that monomers are not linked by disulfide bonds in the dimer form. The purified proteins were characterized as phospholipoglycoproteins. Isoelectric focusing of both VTGs revealed components with isoelectric points ranging from 5.3 to 6.0, suggesting charge heterogeneity. The amino acid composition of both VTGs contains a high proportion of nonpolar amino acids and was similar to those of other teleosts. An antibody developed against carp (Cyprinus carpio) VTG showed cross-reactivity with VTG from both redhorse species. Using this antibody, VTG was detected in plasma and surface mucus of E2B-treated redhorse. This is the most extensive report on purification and characterization of vitellogenin from catostomidid species.©2009 Springer

MALTAIS, D., R.L. ROY, 2009. Purification and partial characterization of vitellogenin from shorthead redhorse (Moxostoma macrolepidotum) and copper redhorse (Moxostoma hubbsi) and detection in plasma and mucus with a heterologous antibody. Fish. Physiol. Biochem., 35: 241-54 .

Vitellogenin (VTG), the egg yolk precursor protein, was purified from plasma of estradiol-3-benzoate (E2B)-treated male shorthead redhorse (Moxostoma macrolepidotum) and immature copper redhorse (Moxostoma hubbsi) by a two-step chromatographic procedure without precipitation. Intact VTGs appeared as dimers with apparent molecular masses, determined by gel filtration, of ˜425 kDa (copper redhorse) and ˜450 kDa (shorthead redhorse). In native polyacrylamide gel electrophoresis (PAGE), dimeric redhorse VTGs appeared as a 520 kDa band. Both VTGs were reduced to a single monomer of ˜150 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and nonreducing conditions, indicating that monomers are not linked by disulfide bonds in the dimer form. The purified proteins were characterized as phospholipoglycoproteins. Isoelectric focusing of both VTGs revealed components with isoelectric points ranging from 5.3 to 6.0, suggesting charge heterogeneity. The amino acid composition of both VTGs contains a high proportion of nonpolar amino acids and was similar to those of other teleosts. An antibody developed against carp (Cyprinus carpio) VTG showed cross-reactivity with VTG from both redhorse species. Using this antibody, VTG was detected in plasma and surface mucus of E2B-treated redhorse. This is the most extensive report on purification and characterization of vitellogenin from catostomidid species.©2008 Springer Science+Business Media B.V.

MALTAIS, D., R.L. ROY, 2007. A lateral flow immunoassay for rapid evaluation of vitellogenin levels in plasma and surface mucus of the copper redhorse (>i>moxostoma hubbsi>/i>). Environ. Toxicol. Chem., 26(8): 1672-1676 .

We tested a lateral flow immunoassay (LFIA) for detecting vitellogenin (VTG) in plasma and surface mucus of copper redhorse, Moxostoma hubbsi, a threatened fish species. The LFIA detected VTG in samples from estradiol-induced fish, though there was no reaction in samples from noninduced individuals. The minimum detection range was 0.08 to 0.60 μg VTG/ml, comparable to other methods. The LFIA has the potential to detect exposure to estrogenic endocrine-disrupting chemicals.©2007 SETAC

ROY, R.L., Y. MORIN, S.C. COURTENAY, P. ROBICHAUD, 2004. Purification of vitellogenin from smooth flounder (Pleuronectes putnami) and measurement in plasma by homologous ELISA. Comp. Biochem. Physiol., Part B, 139: 235-244 .

Male smooth flounder (Pleuronectes putnami) were induced to produce vitellogenin (VTG) by injection of 17β-estradiol (E2). Anion exchange chromatography of precipitated plasma from E2-injected resulted in a single peak consisting of VTG. Smooth flounder VTG has an approximate molecular mass of ˜520 kDa, determined by gel filtration with molecular weight standards. Purified VTG was used to develop a homologous enzyme-linked immunosorbent assay (ELISA). The flounder VTG ELISA is an indirect antigen competition assay with a detection limit of 15 ng·ml-1 and a useful range of 30-950 ng·ml-1 of diluted sample. Intra- and inter-assay precision (as  %CV, n=7) ranged from 1.3 % to 6.0 % and 5.1 %, respectively. The ELISA was evaluated using plasma samples collected from a smooth flounder population captured in the Saint Lawrence Estuary. The ELISA is sensitive enough to differentiate males and non-vitellogenic females from vitellogenic individuals during early vitellogenesis.©2004 Elsevier Inc.

SAUCIER, F.J., R. ROY, D. GILBERT, P. PELLERIN, H. RITCHIE, 2003. Modeling the formation and circulation processes of water masses and sea ice in the Gulf of St. Lawrence. J. Geophys. Res. (C Oceans), 108(C8): 3269-3289 .

ROY, R.L., R.G.C. CAMPBELL, S. PRÉMONT, J. LABRIE, 2000. Geochemistry and toxicity of aluminum in the Saguenay River, Québec, Canada, in relation to discharges from an aluminum smelter. Environ. Toxicol. Chem., 19(10): 2457-2466 .