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Bibliography of the Maurice Lamontagne Institute

Paul ROBICHAUD

ROY, R.L., Y. MORIN, S.C. COURTENAY, P. ROBICHAUD, 2004. Purification of vitellogenin from smooth flounder (Pleuronectes putnami) and measurement in plasma by homologous ELISA. Comp. Biochem. Physiol., Part B, 139: 235-244 .

Male smooth flounder (Pleuronectes putnami) were induced to produce vitellogenin (VTG) by injection of 17β-estradiol (E2). Anion exchange chromatography of precipitated plasma from E2-injected resulted in a single peak consisting of VTG. Smooth flounder VTG has an approximate molecular mass of ˜520 kDa, determined by gel filtration with molecular weight standards. Purified VTG was used to develop a homologous enzyme-linked immunosorbent assay (ELISA). The flounder VTG ELISA is an indirect antigen competition assay with a detection limit of 15 ng·ml-1 and a useful range of 30-950 ng·ml-1 of diluted sample. Intra- and inter-assay precision (as  %CV, n=7) ranged from 1.3 % to 6.0 % and 5.1 %, respectively. The ELISA was evaluated using plasma samples collected from a smooth flounder population captured in the Saint Lawrence Estuary. The ELISA is sensitive enough to differentiate males and non-vitellogenic females from vitellogenic individuals during early vitellogenesis.©2004 Elsevier Inc.

BÉLAND, P., D. MARTINEAU, P. ROBICHAUD, R. PLANTE, R. GREENDALE, 1987. Échouages de mammifères marins sur les côtes du Québec dans l'estuaire et le golfe du Saint-Laurent de 1982 à 1985. Rapp. tech. can. sci. halieut. aquat., 1506, 44 p .

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This report compiles strandings of marine mammals identified by CREP staff from 1982 to 1985 along the shores of southern Québec. Only confirmed reports have been included. There were 132 individual cases, representing ten species; the vast majority (80) occurred from May to September. Less than 15 % of these deaths can be attributed directly to human intervention. Currents and denser settlement are responsible for a larger number of reports from the south shore of the St. Lawrence River and Gulf. The beluga, a resident species, comprises 50 % of all cetacean strandings. Seven rorqual whales stranded above the upper limit of saline waters, including one case over 250 km above this point. Relative to their presumed abundances, there is a bias towards larger species; for example, sperm whales, rarely observed at sea in the Gulf, represent close to 4 % of all deaths reported.